Anti-HSPBAP1 antibody

Anti-HSPBAP1 antibody

• 概述

• 产品描述May play a role in cellular stress response.
• 产品名称Anti-HSPBAP1 antibody
• 分子量54 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human HSPBAP1 151-250/488
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm.
• 其它名称27 KdA heat shock protein-associated protein 1
  HBAP1_HUMAN
  hspbap
  HSPB1-associated protein 1
  HSPBAP1
  PASS1
  Protein associated with small stress protein 1
  protein associating with small stress protein.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  293T Cell(human) Lysate at 40 ug
  Lymph node (mouse) Lysate at 40 ug
  HepG2 Cell(human) Lysate at 40 ug
  Testis (mouse) Lysate at 40 ug
  Primary: Anti- HSPBAP1()at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55kD
  Observed band size: 55 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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