Anti-Osterix antibody
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概述
- 产品描述Transcription factor Sp7, also called Osterix (Osx), is a protein that in humans is encoded by the SP7 gene. It is a member of the Sp family of zinc-finger transcription factors [5] It is highly conserved among bone-forming verterbrate species. It plays a major role, along with Runx2 and Dlx5 in driving the differentiation of mesenchymal precursor cells into osteoblasts and eventually osteocytes. Sp7 also plays a regulatory role by inhibiting chondrocyte differentiation maintaining the balance between differentiation of mesenchymal precursor cells into ossified bone or cartilage. Mutations of this gene have been associated with multiple dysfunctional bone phenotypes in vertebrates. During development, a mouse embryo model with Sp7 expression knocked out had no formation of bone tissue. Through the use of GWAS studies, the Sp7 locus in humans has been strongly associated with bone mass density. [10] In addition there is significant genetic evidence for its role in diseases such as Osteoge nesis imperfecta (OI). In a developing organism, Sp7 serves as one of the most important regulatory shepherds for bone formation. Outside of the context of development, in adult mice ablation of Sp7 led to a lack of new bone formation, highly irregular cartilage accumulation beneath the growth plate and defects in osteocyte maturation and functionality.
- 产品名称Anti-Osterix antibody
- 分子量45 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Osterix 331-431/431
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称Osterix
MGC126598
Osx
Sp 7
Sp7
Sp7 transcription factor
Transcription factor Sp7
Zinc finger protein osterix
SP7_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Embryo(Mouse) lysate at 30ug;
Embryo(Mouse) lysate at 30ug;
Primary: Anti-Osterix at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 45kD
Observed band size : 45kD
Fig3: Sample:Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-Osterixat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
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