Anti-RuBisCO large subunit antibody
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概述
- 产品描述Ribulose-1,5-bisphosphate carboxylase/oxygenase, commonly known by the abbreviations Rubisco, rubisco, RuBPCase, or RuBPco, is an enzyme involved in the first major step of carbon fixation, a process by which atmospheric carbon dioxide is converted by plants and other photosynthetic organisms to energy-rich molecules such as glucose. In chemical terms, it catalyzes the carboxylation of ribulose-1,5-bisphosphate (also known as RuBP). It is probably the most abundant enzyme on Earth. RuBisCO is important biologically because it catalyzes the primary chemical reaction by which inorganic carbon enters the biosphere. While many autotrophic bacteria and archaea fix carbon via the reductive acetyl CoA pathway, the 3-hydroxypropionate cycle, or the reverse Krebs cycle, these pathways are relatively small contributors to global carbon fixation than that catalyzed by RuBisCO. Phosphoenolpyruvate carboxylase, unlike RuBisCO, only temporarily fixes carbon. Reflecting its importance, RuBisCO is the most abundant protein in leaves, accounting for 50% of soluble leaf protein in C3 plants (20–30% of total leaf nitrogen) and 30% of soluble leaf protein in C4 plants (5–9% of total leaf nitrogen).
- 产品名称Anti-RuBisCO large subunit antibody
- 分子量52 KDa
- 种属反应性Arabidopsis thaliana
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from Arabidopsis thaliana Rubisco 151-250/475
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Plastid, chloroplast.
- 其它名称rbcL
RBL_SPIOL
Ribulose bisphosphate carboxylase large chain
RuBisCO-large subunit
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit
ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast) [Arabidopsis lyrata].
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Fig1: Sample:
Gourd leaf Lysate at 40 ug
Lotus leaf Lysate at 40 ug
Primary: Anti-RUBISCO at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
Fig2: Sample: Linden Lysate at 40 ug
Primary: Anti-RUBISCO at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
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