Anti-Secretogranin V antibody
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概述
- 产品描述Secretogranin V may have intracellular functions other than PC2 maturation in certain cells, although some recent work shows a discordancy between mRNA expression and enzymic activity. The mechanism for PC2 maturation appears to involve pro-Secretogranin V binding to inactive pro-PC2 via its polyproline motif (116 PPNPCP 121) in the endoplasmic reticulum and chaperoning pro-PC2 to a later secretory pathway compartment where maturation of PC2 proceeds once pro-Secretogranin V is proteolytically processed by a furin-like convertase into a 21kDa fragment and a C-terminal peptide. It is the C-terminal peptide that potently inhibits maturation of pro-PC2 until the complex is subjected to a decreasing pH gradient along the secretory pathway. It seems likely that mature PC2 then cleaves the CT peptide at its internal lys-lys site. Secretogranin V knockout mice develop a severe Cushings-like phenotype and exhibit multiple metabolic and hormonal abnormalities, indicating that Secretogranin V is required for activation of PC2 in vivo. Conversely, PC2-null mice appear to be viable.
- 产品名称Anti-Secretogranin V antibody
- 分子量17/21 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human PHF21A 1-100/212
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Secreted. Note=Neuroendocrine and endocrine secretory granules.
- 其它名称7B2
7B2 protein
APPG
Neuroendocrine protein 7B2
P7B2
Pituitary polypeptide
Pituitary polypeptide
Prohormone convertase chaperone
SCG 5
SCG5
Secretogranin 5
Secretogranin V 7B2 protein
Secretogranin5
SecretograninV
Secretory granule endocrine protein I
Secretory granule endocrine protein I
Secretory granule neuroendocrine protein 1 7B2 protein
Secretory granule neuroendocrine protein 1
Sg V
SGNE 1
SGNE1
SgV
7B2_HUMAN.
more
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Fig1: Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-Secretogranin V t 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:17/21 kD
Observed band size:21 kD
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-Secretogranin V at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/21 kD
Observed band size: 21 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
