Anti-TREM1 antibody

Anti-TREM1 antibody

• 概述

• 产品描述Triggering receptor expressed on myeloid cells 1 is a protein that in humans is encoded by the TREM1 gene. Monocyte/macrophage- and neutrophil-mediated inflammatory responses can be stimulated through a variety of receptors, including G protein-linked 7-transmembrane receptors (e.g., FPR1), Fc receptors (see MIM 146790), CD14 and Toll-like receptors (e.g., TLR4), and cytokine receptors (e.g., IFNGR1; MIM 107470). Engagement of these receptors can also prime myeloid cells to respond to other stimuli. Myeloid cells express receptors belonging to the Ig superfamily, such as TREM1, or to the C-type lectin superfamily. Depending on their transmembrane and cytoplasmic sequence structure, these receptors have either activating (e.g., KIR2DS1; MIM 604952) or inhibitory functions (e.g., KIR2DL1). In granulocyte cells, TREM1 is activated by C/EBPε independently from inflammatory response. Model organisms have been used in the study of TREM1 function. A conditional knockout mouse line called Trem1tm1(KOMP)Vlcg was generated at the Wellcome Trust Sanger Institute. Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Additional screens performed: - In-depth immunological phenotyping.
• 产品名称Anti-TREM1 antibody
• 分子量24 KDa
• 种属反应性Human,Rat
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human TREM1 151-234/234
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential).
• 其它名称TREM 1
  TREM-1
  Triggering receptor expressed on monocytes 1
  Triggering receptor expressed on myeloid cells 1
  Triggering receptor TREM 1
  Triggering receptor TREM1
  Triggering-receptor TREM 1
  Triggering-receptor TREM-1
  Triggering-receptor TREM1
  TREM1_HUMAN
  CD354.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

•  

  

  Fig1: Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number:Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

   

  

  Fig2: Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature)

   

  

  Fig3: Sample:hcclm3(Human) Cell Lysate at 30 ug
  K562(Human) Cell Lysate at 30 ug
  Primary: Anti-CD200R (bs-7351R) at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 24 kD
  Observed band size: 21 kD

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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