Anti-TRIM21 antibody
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概述
- 产品描述E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules.
- 产品名称Anti-TRIM21 antibody
- 分子量52 KDa
- 种属反应性Human,Mouse
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human SSA N-terminus 301-400/475
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称52 kDa ribonucleoprotein autoantigen Ro/SS-A
52 kDa Ro protein
52kD Ro/SSA autoantigen
Autoantigen Ro/SSA, 52-KD
E3 ubiquitin-protein ligase TRIM21
RING finger protein 81
RNF81
Ro 52
Ro(SS-A)
Ro52
RO52_HUMAN
Sicca syndrome antigen A
Sjoegren syndrome type A antigen
Sjogren syndrome antigen A1
Sjogren syndrome type A antigen
SS-A
SSA
SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro)
TRIM21
Tripartite motif protein TRIM21
Tripartite motif-containing 21
Tripartite motif-containing protein 21.
more
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Fig1: Sample: U937 Cell Lysate at 40 ug
Primary: Anti-TRIM2at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 56 kD
Fig2: Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- TRIM21 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Fig3: Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- TRIM21 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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