Anti-IGF-IR antibody
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概述
- 产品描述This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
- 产品名称Anti-IGF-IR antibody
- 种属反应性Human, Mouse, Rat
- 验证应用IF, WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human IGF1R. AA range:1126-1175
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称IGF1R antibody; Insulin-like growth factor 1 receptor antibody; Insulin-like growth factor I receptor antibody; IGF-I receptor antibody; CD antigen CD221 antibody; INSR antibody; Insulin receptor antibody; IR antibody; CD antigen CD220 antibody;
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应用
IF: 1:50-200 WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Immunofluorescence analysis of rat-kidney tissue. 1,IGF-IR Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Fig2: Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,IGF-IR Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Fig3: Western Blot analysis of various cells using IGF-IR Polyclonal Antibody diluted at 1：2000
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
