Anti-N4BP1 antibody
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概述
- 产品描述PTM:Monoubiquitinated. PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Belongs to the N4BP1 family. subcellular location:In discrete circular structures. subunit:Binds NEDD4. tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
- 产品名称Anti-N4BP1 antibody
- 分子量100 kDa
- 种属反应性Human
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称
- N4BP1 antibody; KIAA0615 antibody; NEDD4-binding protein 1 antibody; N4BP1 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of various cells using N4BP1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Fig2: Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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