Anti-TOP2A antibody
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概述
- 产品描述DNA topoisomerases I and II are nuclear enzymes; type II consists of two highly homologous isoforms: topoisomerase IIα and IIβ. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand(s).
- 产品名称Anti-TOP2A antibody
- 分子量175 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC
- 抗体类型兔多抗
- 免疫原recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus
- 其它名称
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
more
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应用
WB: 1:1,000-1:2,000
ICC: 1:200
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Fig1: Western blot analysis on Jurkat cell lysates using anti-TOP2A rabbit polyclonal antibodies.
Fig2: ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
