Anti-TESPA1 antibody
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概述
- 产品描述TESPA1 (Thymocyte-expressed positive selection-associated protein 1) is required for the development and maturation of T-cells, its function being essential for the late stages of thymocyte development. It plays a role in T-cell antigen receptor (TCR)-mediated activation of the ERK and NFAT signaling pathways, possibly by serving as a scaffolding protein that promotes the assembly of the LAT signalosome in thymocytes.
- 产品名称Anti-TESPA1 antibody
- 分子量50kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) corresponding to the aa 210-250 of human TESPA1.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm. Membrane.
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应用
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:50
FC: 1:50-1:100
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Fig1: Western blot analysis of TESPA1 on different lysates using anti-TESPA1 antibody at 1/1,000 dilution.
Fig2: ICC staining TESPA1 in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-TESPA1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HepG2 cells with TESPA1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondar
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