Anti-UBC9 antibody
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概述
- 产品描述UBC9 is a component of the ubiquitin-mediated proteolytic pathway, which targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization. Ub and Ub-like molecules are systematically transferred from E2 conjugating enzymes to the targeted substrate by way of an E3 ubiquitin ligase. UBC9 functions as an E2 ubiquitin conjugating enzyme that preferentially associates with the ubiquitin homolog designated SUMO-1 or sentrin, a component of the sentrinization complex. Characteristic of the E2 family members, UBC9 contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins and the E2 member, and it shares a conserved UBC domain. Substrates for UBC9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, which indicates that UBC9 may regulate various cellular processes including cell cycle progression and differentiation.
- 产品名称Anti-UBC9 antibody
- 分子量18 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF,IHC-P
- 抗体类型兔多抗
- 免疫原Amino acids 1-81 of UBC9 of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm, Nucleus
- 其它名称
- C358B7.1 antibody
- p18 antibody
- SUMO 1 protein ligase antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
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Fig1: Western blot analysis of UBC9 expression in Jurkat whole cell lysate.
Fig2: Western blot analysis of UBC9 expression in HeLa whole cell lysate.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
