Anti-Msx-2 antibody
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概述
- 产品描述Homeobox protein MSX-2 is a protein that in humans is encoded by the MSX2 gene. The encoded protein is a transcriptional repressor whose normal activity may establish a balance between survival and apoptosis of neural crest-derived cells required for proper craniofacial morphogenesis. The encoded protein may also have a role in promoting cell growth under certain conditions and may be an important target for the RAS signaling pathways. Mutations in this gene are associated with parietal foramina 1 and craniosynostosis type 2. Msx2 is a homeobox gene localized on human chromosome 5 that encodes a transcription repressor and activator (MSX-2) responsible for craniofacial and limb-bud development. Cells will express msx2 when exposed to signaling molecules BMP-2 and BMP-4 in situ. Expression of msx2 leads to the proliferation, migration and osteogenic differentiation of neural crest cells during embryogenesis and bone fracture. It is well documented that expression of cell-cell adhesion molecules such as E-cadherins will promote structural integrity and an epithelial arrangement of cells, while expression of N-cadherin and vimentin promote mesenchymal arrangement and cell migration. Msx2 downregulates E-cadherins and upregulates N-cadherin and vimentin which indicates its role in inducing epithelial mesenchymal transition (EMT).
- 产品名称Anti-Msx-2 antibody
- 分子量28 kDa
- 种属反应性Human,Mouse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原Synthesized peptide derived from the Internal region of human Msx-2.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 亚细胞定位Nucleus
- 其它名称
- CRS 2 antibody
- CRS2 antibody
- FPP antibody
more
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应用
WB:1:500-1:2,000
IHC-P:1:50-1:200
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Fig1: Western blot analysis of Msx-2 on CoLo cells lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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