Anti-SARS-CoV-2 Nucleocapsid Protein antibody
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概述
- 产品描述Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
- 产品名称Anti-SARS-CoV-2 Nucleocapsid Protein antibody
- 分子量48 kDa
- 种属反应性SARS-CoV-2
- 验证应用ELISA,WB
- 抗体类型兔多抗
- 免疫原Full length of SARS-CoV-2 Nucleocapsid Protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Antigen affinity purified.
- 亚细胞定位Cytoplasm of SARS-CoV-2.
- 其它名称
- SARS-CoV-2 Nucleocapsid Protein antibody
- SARS-CoV-2 NP antibody
- nucleocapsid protein antibody
more
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应用
ELISA: 1:1,000-1:5,000
WB:1:500-1:2,000
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Fig1: ELISA analysis with anti-SARS-CoV-2 Nucleocapsid Proein antibody. Antigen (1 μg/mL). The antigen was used as the coating antigen, and the anti-SARS-CoV-2 Nucleocapsid Protein antibody was used as the capture antigen for ELISA.
Fig2: Western blot using anti-SARS-CoV-2 Nucleocapsid Protein antibody shows detection of a 48 kDa band corresponding to the protein.
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