Anti-SRC antibody
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概述
- 产品描述Src (pronounced "sarc" as it is short for sarcoma) is a proto-oncogene encoding a tyrosine kinase. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. c-Src is made up of 6 functional regions: Src homology (SH) 4 domain (SH4 domain), unique region, SH3 domain, SH2 domain, catalytic domain and short regulatory tail. When Src is inactive, the phosphorylated tyrosine group at the 527 position interacts with the SH2 domain which helps the SH3 domain interact with the linker domain and consequently keeps the inactive unit tightly bound. The activation of c-Src causes the dephosphorylation of the tyrosine 527 which causes the structure to be destabilized and then result in the opening up of the SH3, SH2 and the kinase domains and autophosphorylation of tyrosine 416. The activation of the c-Src pathway has been observed in about 50% of tumors from colon, liver, lung, breast and the pancreas.
- 产品名称Anti-SRC antibody
- 分子量60 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cell membrane, nucleus, cytoplasm
- 其它名称
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- ASV antibody
- Avian sarcoma virus antibody
- c SRC antibody
more
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应用
WB: 1:1000-1:2000
ICC: 1:100-1:200
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Fig1: Western blot analysis on cell lysates using anti-SRC rabbit polyclonal antibodies.
Fig2: ICC staining SRC in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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