Anti-OAS3 antibody

Anti-OAS3 antibody

• 概述

• 产品描述The family of EF-hand type Ca2+-binding proteins includes Calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, Cal-granulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins) and the parvalbumin family members, including parvalbumin α and parvalbumin β (also designated oncomodulin). Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues and Calbindin is present in intestine and kidney. Parvalbumin α is also found in fast-contracting/ relaxing skeletal muscle fibers and parvalbumin β is found in many tumor tissues as well as in the organ of Corti. Calbindin, S-100 proteins and parvalbulmins have all been detected in Leydig cells and the testis. These proteins are thought to play a role in hormone production and spermatogenesis. Calgranulin is expressed in macrophages and epithelial cells.
• 产品名称Anti-OAS3 antibody
• 分子量121 kDa
• 种属反应性Human
• 验证应用WB,IHC,ICC
• 抗体类型兔多抗
• 免疫原Recombinant protein within human OAS3 aa 900-1059.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein affinity purified.
• 亚细胞定位Cytoplasm, Nucleus.
• 其它名称
  • 别名
  • (2-5'')oligo(A) synthase 3 antibody
  • 2 5' oligo(A) synthetase 3 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC: 1:100-1:500
  ICC: 1:50-1:200

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  Fig1: Western blot analysis of OAS3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: A549 cell lysate
  Lane 2: Human placental tissue lysate
  Lane 3: A431 cell lysate

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-OAS3 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500039, 1/200 room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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