Anti-HSP60 antibody
-
概述
- 产品描述The 60 kDa heat shock protein (HSP60) is a highly conserved protein that acts as a chaperone involved in correct folding of newly synthesized proteins. Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. The HSP60 proteins are abundant in the cell and are highly conserved between bacteria and human, with 50%-61% sequence homology.
- 产品名称Anti-HSP60 antibody
- 分子量61kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, ICC, IHC-P
- 抗体类型小鼠单抗
- 免疫原peptide
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Mitochondrion matrix
- 其它名称
- 60 kDa chaperonin antibody
- 60 kDa heat shock protein, mitochondrial antibody
- CH60_HUMAN antibody
more
-
应用
WB: 1:2,000
ICC: 1:200
IHC-P: 1:200
-
Fig1: Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Jurkat
Lane 4: PC12
Lane 5: F9
Fig2: ICC staining HSP60 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
