Anti-GPC3 antibody [G3-C2]

Anti-GPC3 antibody [G3-C2]

• 概述

• 产品描述Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
• 产品名称Anti-GPC3 antibody [G3-C2]
• 分子量65 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Plasma membrane. Secreted.
• 其它名称
  • DGSX antibody
  • Glypican proteoglycan 3 antibody
  • Glypican-3 [Precursor] antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Glypican3 on human Glypican3 recombinant protein using anti- Glypican3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Glypican3 on different lysates using anti- Glypican3 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: HEK293
  Lane 3:Jurkat
  Lane 4: SK-N-SH
  Lane 5: PC-12
  Lane 6: F9
  Lane 7: Mouse liver tissue

   

  

  Fig3: ICC staining Glypican3 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded liver cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti- Glypican3 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Jurkat cells with Glypican3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！