Anti-TWIST1 antibody [E6-F4]

Anti-TWIST1 antibody [E6-F4]

• 概述

• 产品描述Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome.
• 产品名称Anti-TWIST1 antibody [E6-F4]
• 分子量25 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • ACS3 antibody
  • B-HLH DNA binding protein antibody
  • bHLHa38 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of TWIST1 on human TWIST1 recombinant protein using anti-TWIST1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of TWIST1 on HEK293 (1) and TWIST1-hIgGFc transfected HEK293 (2) cell lysate using anti-TWIST1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of TWIST1 on different cell lysates using anti-TWIST1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: NIH/3T3
  Lane 2: JURKAT
  Lane 3: HELA
  Lane 4: A549
  Lane 5: RAJI
  Lane 6: OCM-1

   

  

  Fig4: ICC staining TWIST1 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded colon cancer tissue using anti-TWIST1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with TWIST1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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