Anti-CCL2 antibody [E11-D8]

Anti-CCL2 antibody [E11-D8]

• 概述

• 产品描述Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
• 产品名称Anti-CCL2 antibody [E11-D8]
• 分子量15 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted.

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CCL2 on human CCL2 recombinant protein using anti-CCL2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CCL2 on different cell lysates using anti-CCL2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: A549
  Lane 2: HELA
  Lane 3: Raw264.7
  Lane 4: L1210
  Lane 5: C6
  Lane 6: Cos7

   

  

  Fig3: ICC staining CCL2 (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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