Anti-CFLAR antibody [D12-A3]

Anti-CFLAR antibody [D12-A3]

• 概述

• 产品描述FLIP (FLICE inhibitory protein) is expressed as both long and short forms and is involved in the regulation of apoptosis. The short form of FLIP contains two death effector domains homologous to the death effector domain of the FAS-associating protein FADD. The long form of FLIP, which shares significant homology with the cysteine protease FLICE, contains an additional caspase-like domain, but lacks a catalytic active site and lacks the residues that form the substrate binding pocket in most caspases. FLIP has been designated by independent groups as Casper, I-FLICE, CLARP, FLAME-1 and MRIT. Although its exact role is still being elucidated, FLIP appears to be an important factor in the regulation of apoptosis downstream of all known death receptors.
• 产品名称Anti-CFLAR antibody [D12-A3]
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • c FLIP antibody
  • c FLIPL antibody
  • c FLIPR antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CFLAR on human CFLAR recombinant protein using anti-CFLAR antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CFLAR on different cell lysate using anti-CFLAR antibody at 1/1,000 dilution.
  Positive control: Line1: Jurkat Line2: 3T3L1 Line3: Raji

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-CFLAR antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Jurkat cells with CFLAR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！