Anti-Pirh2 antibody [D6-H10]

Anti-Pirh2 antibody [D6-H10]

• 概述

• 产品描述Pirh2, also known as Androgen receptor N-terminal-interacting protein (ARNIP), ZN363 or CHIMP, has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. The protein physically interacts with p53 and the resulting degradation of p53 renders Pirh2 an oncogenic protein as the loss of p53 function contributes to malignant tumor development. The gene encoding for the protein maps to chromosome 4q21.1 and transcription of this gene is regulated by p53. Pirh2 expression decreases the level of p53 and a decrease of endogenous Pirh2 expression ups p53 levels. Pirh2 is therefore considered, together with MDM2, to be acting as a negative regulator of p53 function.
• 产品名称Anti-Pirh2 antibody [D6-H10]
• 分子量30 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Nucleus speckle. Cytoplasm.
• 其它名称
  • Androgen receptor N terminal interacting protein antibody
  • Androgen receptor N-terminal-interacting protein antibody
  • ARNIP antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Pirh2 on different cell lysate using anti-Pirh2 antibody at 1/1,000 dilution.
  Positive control:
  Line1: Hela Line2: A549
  Line3: MCF-7 Line4: PC-12

   

  

  Fig2: ICC staining Pirh2 (green) and Actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Pirh2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of PC-12 cells with Pirh2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！