Anti-DDX3X antibody

Anti-DDX3X antibody

• 概述

• 产品描述DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 (DEAD box polypeptide 3) is involved in RNA metabolism. Two DDX3 paralogs are found in humans; DDX3X is encoded by a gene found on the X chromosome while DDX3Y is encoded by a gene on the Y chromosome. DDX3Y is exclusively expressed in testis and is required for normal spermatogenesis. DDX3X is ubiquitously expressed and predominantly localizes to the nuclear speckles, participating in RNA splicing, transcription, translation initiation, mRNA transport and cell cycle regulation. DDX3X also partakes in HIV-1 replication and hepatitis C viral infections.
• 产品名称Anti-DDX3X antibody
• 分子量73/71 kDa
• 种属反应性Human,Mouse,Monkey,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus speckle. Cytoplasm.
• 其它名称
  • ATP dependent RNA helicase DDX3X antibody
  • ATP-dependent RNA helicase DDX3X antibody
  • CAP Rf antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of DDX3X on human DDX3X recombinant protein using anti-DDX3X antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of DDX3X on HEK293 (1) and DDX3X-hIgGFc transfected HEK293 (2) cell lysate using anti-DDX3X antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of DDX3X on different cell lysate using anti-DDX3X antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: NIH3T3
  Lane 3: C6
  Lane 4: COS7
  Lane 5: A431
  Lane 6: HEK293

   

  

  Fig4: ICC staining DDX3X (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DDX3X antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

   

  

  Fig8: Flow cytometric analysis of HepG2 cells with DDX3X antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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