Anti-CFHR5 antibody
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概述
- 产品描述Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).
- 产品名称Anti-CFHR5 antibody
- 分子量64 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2a
- 纯化方式Protein A purified.
- 亚细胞定位Secreted.
- 其它名称
- CFHL5 antibody
- CFHR5 antibody
- CFHR5D antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
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Fig1: Western blot analysis of CFHR5 on human CFHR5 recombinant protein using anti-CFHR5 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of CFHR5 on HEK293 (1) and CFHR5-hIgGFc transfected HEK293 (2) cell lysate using anti-CFHR5 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of CFHR5 on different cell lysate using anti-CFHR5 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: K562
Lane 3: L-02
Lane 4: SK-Hep-1
Lane 5: SMMC-7721
Lane 6: NIH/3T3
Fig4: ICC staining CFHR5 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CFHR5 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CFHR5 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with CFHR5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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