Anti-CD2 antibody [B4-D1]

Anti-CD2 antibody [B4-D1]

• 概述

• 产品描述CD2 (also designated E-rosette receptor) interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific T cell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes, as well as on mouse B cells and rat splenic macrophages. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counterreceptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
• 产品名称Anti-CD2 antibody [B4-D1]
• 分子量55 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane
• 其它名称
  • CD 2 antibody
  • CD2 antibody
  • CD2 antigen (p50), sheep red blood cell receptor antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:100-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of CD2 on human CD2 recombinant protein using anti-CD2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD2 on HEK293 (1) and CD2-hIgGFc transfected HEK293 (2) cell lysate using anti-CD2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CD2 on different cell lysate using anti-CD2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: MOLT4
  Lane 2: MCF-7
  Lane 3: Hela
  Lane 4: L1210

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with CD2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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