Anti-MMP2 antibody [B11-E6]

Anti-MMP2 antibody [B11-E6]

• 概述

• 产品描述The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
• 产品名称Anti-MMP2 antibody [B11-E6]
• 分子量72 kDa
• 种属反应性Human,Mouse
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted. Membrane. Nucleus
• 其它名称
  • 72 kDa gelatinase antibody
  • 72kD type IV collagenase antibody
  • CLG 4 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MMP2 on human MMP2 recombinant protein using anti-MMP2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MMP2 on HEK293 (1) and MMP2-hIgGFc transfected HEK293 (2) cell lysate using anti-MMP2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of MMP2 on different cell lysate using anti-MMP2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: MCF-7
  Lane 2: Raw264.7
  Lane 3: HUVEC
  Lane 4: T47D

   

  

  Fig4: Flow cytometric analysis of Hela cells with MMP-2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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