Anti-CHRNA5 antibody [D10-D2]

Anti-CHRNA5 antibody [D10-D2]

• 概述

• 产品描述Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα5, also known as LNCR2 or CHRNA5 (cholinergic receptor, nicotinic, alpha 5), is a 468 amino acid multi-pass membrane protein belonging to the ligand-gated ionic channel family and is involved in the mediation of fast signal transmission at synapses.
• 产品名称Anti-CHRNA5 antibody [D10-D2]
• 分子量53 kDa
• 种属反应性Human,Rat
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell junction, synapse, postsynaptic cell membrane. Cell membrane
• 其它名称
  • Acetylcholine receptor nicotinic alpha 5 (neuronal) antibody
  • ACHA5_HUMAN antibody
  • AChR antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:50-1:100

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  Fig1: Western blot analysis of CHRNA5 on human CHRNA5 recombinant protein using anti-CHRNA5 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CHRNA5 on HEK293 (1) and CHRNA5-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CHRNA5 on different cell lysate using anti-CHRNA5 antibody at 1/1,000 dilution.
  Positive control: Line1: membrane protein lysate of C6 Line2: membrane protein lysate of SK-N-SH Line3: membrane protein lysate of C6 Line4: C6 cell lysate

   

  

  Fig4: Flow cytometric analysis of SK-N-SH cells with CHRNA5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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