Anti-RPS6KA2 antibody [8C-4C]

Anti-RPS6KA2 antibody [8C-4C]

• 概述

• 产品描述The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development. The family of ribosomal S6 kinases includes p70 S6 kinase and p70 S6 kinase β, which are thought to have similar regulatory functions. MSK1 (also designated RLPK) is a novel Rsk-related protein, which, like the p90 Rsk family members, contains two non-identical complete kinase catalytic domains.
• 产品名称Anti-RPS6KA2 antibody [8C-4C]
• 分子量83 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, cytoplasm.
• 其它名称
  • 90 kDa ribosomal protein S6 kinase 2 antibody
  • HU 2 antibody
  • KS6A2_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of RPS6KA2 on human RPS6KA2 recombinant protein using anti-RPS6KA2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of RPS6KA2 on HEK293 (1) and RPS6KA2-hIgGFc transfected HEK293 (2) cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of RPS6KA2 on different cell lysate using anti-RPS6KA2 antibody at 1/1,000 dilution.
  Positive control: Line1: Hela Line2: A431 Line3: HEK293 Line4: Jurkat Line5: HepG2 Line6: MCF-7 Line7: NIH/3T3

   

  

  Fig4: ICC staining RPS6KA2 (green) and Actin filaments (red) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RPS6KA2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with RPS6KA2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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