Anti-RICTOR antibody [E9-B3]

Anti-RICTOR antibody [E9-B3]

• 概述

• 产品描述Rapamycin-insensitive companion of FRAP, also designated Rictor, shares homology with pianissimo from D. discoidieum, STE20p from S . pombe, and AVO3p from S. cerevisiae. Rictor forms a complex with FRAP, which is important in cell growth regulation as it integrates growth factor and nutrient derived signals. The Rictor-FRAP complex plays a role in PKC alpha phosphorylation, directly phosphorylates Akt / PKB on Ser473 in vitro and facilitates Thr308 phosphorylation by PDK1. It also may function as a drug target in tumors that have lost expression of PTEN, a tumor suppressor that opposes activation of Akt / PKB.
• 产品名称Anti-RICTOR antibody [E9-B3]
• 分子量192 kDa
• 种属反应性Human,Mouse,Monkey
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol.
• 其它名称
  • AVO3 antibody
  • AVO3 homolog antibody
  • DKFZp686B11164 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of RICTOR on different cell lysates using anti-RICTOR antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: PANC-1
  Lane 3: MOLT4
  Lane 4: HepG2
  Lane 5: HEK293

   

  

  Fig2: ICC staining RICTOR (green) and actin filaments (red) in NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-RICTOR antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RICTOR antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with RICTOR antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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