Anti-γ-IFN antibody [AH25-12]

Anti-γ-IFN antibody [AH25-12]

• 概述

• 产品描述IFN-γ induces a variety of biological responses, such as antiviral, antiproliferative and immunomodulatory activity in sensitive cells. Activation of the IFN-γ receptor (IFN-γR) leads to autophosphorylation of the Janus kinases JAK1 and JAK2, and the nuclear translocation of the transcription factors Stat1α p91 and Stat1β p84. The IFN-γR is composed of at least two chains, designated IFN-γRα and IFN-γRβ, respectively. Although expression of IFN-γRα is sufficient for ligand binding, it alone does not confer responsiveness to IFN-γ. Concomitant expression of IFN-γRα and IFN-γRβ is required for transcriptional activation of IFN-γ-inducible genes. The IFN-γRβ chain, also called AF-1, is 332 and 337 amino acids in length in mouse and human, respectively, and may represent the signal transducing component of the IFN-γR.
• 产品名称Anti-γ-IFN antibody [AH25-12]
• 分子量15 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用ELISA,WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at -20 ℃. Stable for 12 months from date of receipt.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.13% Sodium Azide.
• 亚型IgG1
• 亚细胞定位Secreted.
• 其它名称
  • IFG IFI IFN gamma IFN Immune IFNG Interferon gamma Interferon Gamma Precursor Macrophage Activating Factor MAF T Cell Interferon Type II Interferon

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  ELISA: 1:5,000-1:10,000

•  

  

  Fig1: ICC staining γ-IFN in 239T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining γ-IFN in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

   

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse tsetis tissue using anti-γ-IFN antibody. Counter stained with hematoxylin.

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