Anti-Neuron-specific class III β-tubulin antibody [A8-D10]

Anti-Neuron-specific class III β-tubulin antibody [A8-D10]

• 概述

• 产品描述Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
• 产品名称Anti-Neuron-specific class III β-tubulin antibody [A8-D10]
• 分子量50 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide (KLH-coupled) within human/mouse 1-50 aa.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Peptide affinity purified.
• 亚细胞定位Cytoplasm. Cytoskeleton. Microtubule.
• 其它名称
  • beta 3 tubulin antibody
  • beta-4 antibody
  • CDCBM antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Neuron-specific class III b-tubulin on Hela using anti-Neuron-specific class III b-tubulin antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining Neuron-specific class III b-tubulin (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Neuron-specific class III b-tubulin (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neuron-specific class III b-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of MCF-7 cells with Neuron-specific class III b-tubulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse

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