Anti-Citrate synthase antibody [1G1]

Anti-Citrate synthase antibody [1G1]

• 概述

• 产品描述Citrate synthase (CS) is a 466 amino acid mitochondrial matrix protein that functions as the first and rate-limiting enzyme of the tricarboxylic acid cycle. Essential in mitochondrial respiration and involved in the conversion of glucose to lipid, citrate synthase is found the great majority of cells that are capable of oxidative metabolism. The gene encoding citrate synthase maps to human chromosome 12q13.3, which is transcribed into two alternatively spliced variants designated CSa and CSb. Human chromosome 12 encodes over 1,100 genes, comprises approximately 4.5% of the human genome and is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders.
• 产品名称Anti-Citrate synthase antibody [1G1]
• 分子量52kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原An epitope mapping between amino acids 259-292 of citrate synthase of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion matrix
• 其它名称
  • CISY_HUMAN antibody
  • Citrate synthase antibody
  • Citrate synthase, mitochondrial antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500
  IHC-P: 1:50-500

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  Fig1: Western blot analysis of citrate synthase expression in non-transfected 293T (A), mouse citrate synthase transfected 293T (B) and K-562 (C) whole cell lysates.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes (B).

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