Anti-HIF-1 alpha antibody [5H7]

Anti-HIF-1 alpha antibody [5H7]

• 概述

• 产品描述Cell growth and viability is compromised by oxygen deprivation (hypoxia).Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1),EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes,thereby activating transcription. Hypoxia-inducible expression of some genes,such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
• 产品名称Anti-HIF-1 alpha antibody [5H7]
• 分子量132 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, IP, ICC, IHC-P
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃.
• 存储缓冲液1*TBS (pH7.4), 1%BSA. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus, Nucleus speckle
• 其它名称
  • ARNT interacting protein antibody
  • ARNT-interacting protein antibody
  • Basic helix loop helix PAS protein MOP1 antibody
  more

• 应用

  WB: 1:1,000
  IP: 1-2 μg per 100-500 μg of total protein
  ICC: 1:50-500
  IHC-P: 1:50-500

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  Fig1: A.Western blot analysis of HIF-1 expression in non-transfected (A) and mouse HIF-1 transfected (B) 293T whole cell lysates.

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