Anti-MDM2 antibody [3G2]

Anti-MDM2 antibody [3G2]

• 概述

• 产品描述p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.
• 产品名称Anti-MDM2 antibody [3G2]
• 分子量55/90 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用WB, IP, IF, IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 154-167 of MDM2 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus
• 其它名称
  • ACTFS antibody
  • Double minute 2 protein antibody
  • E3 ubiquitin-protein ligase Mdm2 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

•  

  

  Fig1: Western blot analysis of MDM2 expression in U-2OS (A), A-673 (B) and RAW 264.7 (C) whole cell lysates.

   

  

  Fig2: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (A). Immunofluorescence staining of methanol-fixed rat embryo fibroblasts showing nuclear localization (B).

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