Anti-PP2A(alpha+beta) antibody [A0-G7]

Anti-PP2A(alpha+beta) antibody [A0-G7]

• 概述

• 产品描述PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.
• 产品名称Anti-PP2A(alpha+beta) antibody [A0-G7]
• 分子量35 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • PP2A A antibody
  • PP2A alpha antibody
  • PP2A B antibody
  more

• 应用

  WB: 1:2,000-1:5,000
  ICC: 1:100-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.

   

  

  Fig2: ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Flow cytometric analysis of Hela cells with PP2A(alpha+beta) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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