Anti-Phospho-p70 S6 Kinase (Ser 411) antibody [2G1]

Anti-Phospho-p70 S6 Kinase (Ser 411) antibody [2G1]

• 概述

• 产品描述In studies to elucidate key regulatory pathways in signal transduction, several protein serine/threonine (Ser/Thr) kinases have been identified. Included among such kinases are two distinct families of 40S ribosomal protein S6 Ser/Thr kinases present in somatic animal cells, designated p70 S6 kinase and p90 Rsk kinase. p90 Rsk kinase is maximally activated within minutes of addition of growth factors or phorbol ester to cultured cells followed by activation of p70 S6 kinase. Both enzymes are regulated by serine/threonine phosphorylation, suggesting that specific kinases may exist upstream in the signaling pathway that regulate these kinases. In fact, evidence suggests that one such family of activating enzymes includes the members of the ERK MAP kinase family. The ERK MAP kinases are, in turn, regulated by phosphorylation at threonine and tyrosine residues by a protein kinase designated MEK.
• 产品名称Anti-Phospho-p70 S6 Kinase (Ser 411) antibody [2G1]
• 分子量70kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原A sequence containing Ser 411 phosphorylated p70 S6 kinase α isoform 2 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus
• 其它名称
  • 70 kDa ribosomal protein S6 kinase 2 antibody
  • EC 2.7.11.1 antibody
  • KS6B2_HUMAN antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

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  Fig1: Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin treated and lambda protein phosphatase treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (A-6) (A,B,C) and p70 S6 kinase α (C-18) (D,E,F).

   

  

  Fig2: Immunofluorescence staining of anisomycin-treated, methanol-fixed NIH/3T3 cells, showing cytoplasmic and nuclear localization of activated p70 S6 kinase α (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells (B).

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