Anti-CD44 antibody [A7-1]

Anti-CD44 antibody [A7-1]

• 概述

• 产品描述CD44 is a cell-surface glycoprotein postulated to play a role in a variety of biological processes, including lymphocyte homing and tumor-cell metastasis. It mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA), and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications.
• 产品名称Anti-CD44 antibody [A7-1]
• 分子量80 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Peptide affinity purified
• 亚细胞定位Cell membrane
• 其它名称
  • LHR antibody
  • BA-1 antibody
  • CD 44 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD44 on different cell lysates using anti- CD44 antibody at 1/500 dilution.
  Positive control: Line1: HUVEC Line2: HL-60 Line3: Hela Line4:SKOV3

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！