Anti-PRKACG antibody [B3-E4]

Anti-PRKACG antibody [B3-E4]

• 概述

• 产品描述Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated PKAα cat (Cα), PKAβ cat (Cβ) and PKAγ cat (Cγ). Each subunit represents specific gene products. PKAα cat and PKAβ cat are closely related (93% amino acid sequence similarity), whereas PKAγ cat displays 83% and 79% similarity to PKAα cat and PKAβ cat, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on Serine 133, which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the Pol II transcription initiation complex.
• 产品名称Anti-PRKACG antibody [B3-E4]
• 分子量40 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosol. Nucleus.
  
• 其它名称
  • BDPLT19 antibody
  • cAMP dependent protein kinase alpha catalytic subunit antibody
  • cAMP dependent protein kinase beta catalytic subun antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PRKACG on human PRKACG recombinant protein using anti-PRKACG antibody at 1/1,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PRKACG antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-PRKACG antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of MCF-7 cells with PRKACG antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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