Anti-ITK antibody [A6-G12]

Anti-ITK antibody [A6-G12]

• 概述

• 产品描述The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. Four alternatively spliced forms of Tec are found to be expressed broadly in cells Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.
• 产品名称Anti-ITK antibody [A6-G12]
• 分子量72 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • EMT antibody
  • Homolog of mouse T cell itk/tsk antibody
  • IL 2 inducible T cell kinase antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ITK on human ITK recombinant protein using anti-ITK antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ITK on Jurkat cell lysate using anti-ITK antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining ITK (green) in Jurkat cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Jurkat cells with ITK antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

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