Anti-TUBA8 antibody [D7-D6]

Anti-TUBA8 antibody [D7-D6]

• 概述

• 产品描述Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and e Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and e Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell-cycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes.
• 产品名称Anti-TUBA8 antibody [D7-D6]
• 分子量50 kDa
• 种属反应性Human,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Cytoskeleton.
• 其它名称
  • Alpha tubulin 8 antibody
  • Alpha-tubulin 8 antibody
  • TBA8_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of TUBA8 on human TUBA8 recombinant protein using anti-TUBA8 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of TUBA8 on at heart tissue lysate using anti-TUBA8 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining TUBA8 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using anti-TUBA8 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of NIH/3T3 cells with TUBA8 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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