Anti-6X His tag (C terminal) antibody [8-F0]

Anti-6X His tag (C terminal) antibody [8-F0]

• 概述

• 产品描述A variety of plasmids contain DNA that encodes a tag consisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography. This antibody can be used to detect the carboxyl-terminal 6×his tag in recombinant protein.
• 产品名称Anti-6X His tag (C terminal) antibody [8-F0]
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide immune sequence is CHHHHHHHH.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol.
• 亚型IgG2a
• 纯化方式Protein G affinity purified.
• 其它名称
  • 6 His epitope tag antibody
  • Hexa His tag antibody
  • HHHHHH epitope tag antibody
  more

• 应用

  WB: 1:2,000

•  

  

  Fig1: Western blot analysis of 6X His tag (C terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: C-terminal His-tagged recombinant protein
  Lane 2: N-terminal His-tagged recombinant protein

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