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Anti-GCNF antibody [A10-G8]
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:100-1:500
FC: 1:100-1:200
Fig1: Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.
Fig2: Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.
Fig3: Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: NTERA-2
Lane 3: HEK293
Lane 4: HUVE-12
Lane 5: HeLa
Fig4: ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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