Anti-MELK antibody [B7-G2]

Anti-MELK antibody [B7-G2]

• 概述

• 产品描述Maternal embryonic leucine zipper kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNA-binding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.
• 产品名称Anti-MELK antibody [B7-G2]
• 分子量75kDa
• 种属反应性Human
• 验证应用ELISA,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • AI327312 antibody
  • hMELK antibody
  • HPK 38 antibody
  more

• 应用

  ICC: 1:200-1:1000
  IHC-P: 1:200-1:1,000
  FC: 1:100-1:200

•  

  

  Fig1: ICC staining MELK (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-MELK antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of MCF-7 cells with MELK antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red)

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