Anti-PRDX2 antibody [7F4]

Anti-PRDX2 antibody [7F4]

• 概述

• 产品描述The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.
• 产品名称Anti-PRDX2 antibody [7F2]
• 分子量22 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型       小鼠单抗
• 免疫原Recombinant protein.
• 偶联Non-conjugated

• 应用

  • WB: 1:2,000-1:10,000
    ICC: 1:100
    FC: 1:50-1:100

   

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    Fig1: Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti- Peroxiredoxin 2 antibody at 1/5,000 dilution.

   

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    Fig2: ICC staining Peroxiredoxin 2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

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    Fig3: ICC staining Peroxiredoxin 2 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

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    Fig4: Flow cytometric analysis of PC-3M cells with Peroxiredoxin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

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