Anti-EIF5A antibody [6E-F10]

Anti-EIF5A antibody [6E-F10]

• 概述

• 产品描述In mammalian cells, translation is controlled at the level of polypeptide chain initiation by eukaryotic initiation factors. The human eukaryotic translation initiation factor 5A gene, also designated eIF-4D or eIF5A1, maps to chromosome 17p131 and encodes a 154 amino acid protein that is linked to cellular polyamine homeostasis. eIF5A1 localizes to the nuclear and cytoplasmic compartments of mammalian cells where it can stimulate ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport and/or protein translation. eIF5A1 contains a unique spermidine-derived post-translational modification at Lys-50, hypusine, which is necessary for eIF5A1's biochemical activity and for cellular proliferative signaling. In addition, eIF5A1 is a cellular cofactor for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). Inhibition of eIF5A1 interaction with Rev leads to a block of the viral replication cycle. A highly-conserved protein that is found in all living organisms, eIF5A2 (eukaryotic translation initiation factor 5A-2) is a 153 amino acid protein that has 94% sequence similarity to eIF5A1 and also shares the hypusine residue. Amplification of the gene encoding eIF5A2 is observed in ovarian carcinomas and overexpression of eIF5A2 is linked to advanced stages of ovarian cancers.
• 产品名称Anti-EIF5A antibody [6E-F10]
• 分子量16.8 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC,ELISA
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus, Endoplasmic reticulum membrane
• 其它名称
  • eIF 4D antibody
  • eIF 5A 1 antibody
  • EIF 5A antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1:500
  FC: 1:200-1:400
  ELISA: 1:10,000

•  

  

  Fig1: Western blot analysis of EIF5A on human EIF5A recombinant protein using anti-EIF5A antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of EIF5A on HEK293 (1) and EIF5A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF5A antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EIF5A antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with EIF5A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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