Anti-EZH2 antibody [6F-4G]

Anti-EZH2 antibody [6F-4G]

• 概述

• 产品描述In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG), which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. The gene encoding human ENX-1 transcribes a 746 amino acid polypeptide which contains a trithorax-like domain and a DNA-binding motif. ENX-1 interacts with the proto-oncogene product Vav and is thought to be involved in the proliferation of normal and malignant hematopoietic cells. By altering the regulation of target genes, ENX-1 may also contribute to certain phenotypes of Down syndrome.
• 产品名称Anti-EZH2 antibody [6F-4G]
• 分子量86 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • Enhancer of zeste 2 antibody
  • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
  • Enhancer of zeste homolog 2 (Drosophila) antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of EZH2 on human EZH2 recombinant protein using anti-EZH2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of EZH2 on HEK293 (1) and EZH2-hIgGFc transfected HEK293 (2) cell lysate using anti-EZH2 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-EZH2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EZH2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with EZH2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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