Anti-CD49C antibody [5F1D4]

Anti-CD49C antibody [5F1D4]

• 概述

• 产品描述The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function as cell surface adhesion molecules. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 3 subunit. This subunit joins with a beta 1 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. Expression of this gene may be correlated with breast cancer metastasis.
• 产品名称Anti-CD49C antibody [5F1D4]
• 分子量116.6kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human CD49C (AA: extra 63-248) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Membrane.
• 其它名称
  • AA407068 antibody
  • Antigen identified by monoclonal antibody J143 antibody
  • CD49 antigen like family member C antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD49C against human CD49C (AA: extra 63-248) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of against HEK293 (1) and CD49C (AA: extra 63-248)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of against HepG2 (1), HUVEC (2), and HUVE-12 (3) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig4: Flow cytometric analysis of CD49C was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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