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Anti-CD352 antibody [2F1C9]

Anti-CD352 antibody [2F1C9]
别名: CD352
适用物种: Human  
验证应用: WB,IHC-P,FC  
种属: 小鼠单抗  
储存条件: -20℃ 
Anti-CD352 antibody [2F1C9]
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY6711-32M-50 μl 小鼠单抗 现货 1500.00 1500
ZY6711-32M-100 μl 小鼠单抗 现货 2500.00 2500
熔点:
密度:
储存条件: -20℃

 

Anti-CD352 antibody [2F1C9]

 

  • 概述

  • 产品描述The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
  • 产品名称Anti-CD352 antibody [2F1C9]
  • 分子量37.4kDa
  • 种属反应性Human
  • 验证应用WB,IHC-P,FC
  • 抗体类型小鼠单抗
  • 免疫原Purified recombinant fragment of human CD352 (AA: extra 22-226) expressed in E. Coli.
  • 偶联Non-conjugated
  • 性能

  • 形态Liquid
  • 浓度1 mg/mL
  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液1*PBS with 0.05% sodium azide.
  • 亚型IgG1
  • 纯化方式Protein G purified.
  • 亚细胞定位Cell membrane.
  • 其它名称
    • Activating NK receptor antibody
    • CD352 antibody
    • KALI antibody
    more
  • 应用

    WB: 1:500-1:2,000
    IHC-P: 1:50-1:200
    FC: 1:100-1:200

  •  

    Fig1: Western blot analysis of CD352 against human CD352 (AA: extra 22-226) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

     

    Fig2: Western blot analysis of E against HEK293 (1) and CD352 (AA: extra 22-226)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

     

    Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CD352 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

     

    Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD352 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

     

    Fig5: Flow cytometric analysis of CD352 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody  (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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