Anti-RAD23A antibody [3E-4F]

Anti-RAD23A antibody [3E-4F]

• 概述

• 产品描述Mammalian cells express two Rad23 (genome repair protein) homologs, Rad23A (also designated HR23A) and Rad23B (also designated HR23B). In typical cells, mouse Rad23B is approximately ten times more abundant than mouse Rad23A. Endogenous XPC (xeroderma pigmentosum C protein) located in wildtype mouse embryonic fibroblasts is relatively stable; its steady-state level and stability appear to be significantly reduced by a targeted interruption of the mouse Rad23B gene, but not by that of mouse Rad23A. Loss of both mouse Rad23 genes causes a strong further reduction of the XPC protein level. The RAD23 genes (RAD23A and RAD23B), which encode the human Rad23 proteins, are crucial for excision-repair of UV-damaged DNA. RAD23 genes resemble the other DNA repair genes, RAD2, RAD6, RAD7, RAD18 and RAD54, all of which also exhibit increased transcription in response to DNA damage and during meiosis. Rad23 is a nuclear protein containing an ubiquitin-like domain required for biological functions. The protein, which is highly conserved, is involved in nucleotide excision repair (NER) that associates with the proteasome via its N-terminus. The C-terminal ubiquitin-associated domain of Rad23 is evolutionarily conserved from yeast to humans. Rad23 may also act as a regulator for the activity of the 26S Proteasome.
• 产品名称Anti-RAD23A antibody [3E-4F]
• 分子量40 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • hHR 23A antibody
  • hHR23A antibody
  • HR23A antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: Western blot analysis of RAD23A on human RAD23A recombinant protein using anti-RAD23A antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of RAD23A on HEK293 (1) and RAD23A)-hIgGFc transfected HEK293 (2) cell lysate using anti-RAD23A antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-RAD23A antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with RAD23A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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