Anti-WNT3A antibody [EG-64]
-
概述
- 产品描述The Wnt gene family encodes secreted signaling molecules that bind to frizzled receptors and influence oncogenesis and developmental processes, including regulation of cell fate and patterning during embryogenesis. The Wnt family has two functional classes according to their biological activities; Wnts that signal through a Wnt-1/wingless pathway by stabilizing cytoplasmic beta-catenin, and Wnts that stimulate intracellular Ca2+ release and activate two kinases, CamKII and PKC, in a G protein-dependent manner. Wnt-3a is an intercellular signaling molecule that mediates cytoskeletal reorganization and regulates hippocampal development. Human Wnt-3a is 96% homologous to mouse Wnt-3a protein and 84% homologous to human Wnt-3 protein. The human Wnt-3a gene clusters with the Wnt-14 gene at chromosome 1q42.
- 产品名称Anti-WNT3A antibody [EG-64]
- 分子量60 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2b
- 纯化方式Protein A purified.
- 亚细胞定位Secreted, extracellular space, extracellular matrix
- 其它名称
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
more
-
应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of WNT3A on human WNT3A recombinant protein using anti-WNT3A antibody at 1/1,000 dilution.
Fig2: Western blot analysis of WNT3A on HEK293 (1) and WNT3A-hIgGFc transfected HEK293 (2) cell lysate using anti-WNT3A antibody at 1/1,000 dilution.
Fig3: Western blot analysis of WNT3A on different cells lysate using anti-WNT3A antibody at 1/1,000 dilution.
Positive control: Line1: A549 Line2: HepG2 Line3: A431
Fig4: ICC staining WNT3A (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-WNT3A antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with WNT3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
![Anti-WNT3A antibody [EG-64]](images/202012/goods_img/92586_G_1607496387287.jpg)
