Anti-MMP14 antibody [11A-1D]

Anti-MMP14 antibody [11A-1D]

• 概述

• 产品描述The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. Membrane-type matrix metalloproteinases, including MT-MMP-1 (also designated MMP-14), MT-MMP-2 (also designated MMP-15), MT-MMP-3 (also designated MMP-16) and MT-MMP-4 (also designated MMP-17) are type I membrane proteins that function to activate other MMPs. MT-MMP activation appears to be mediated by members of the proprotein convertase family, suggesting that a proprotein convertase/MT-MMP/MMP cascade may be involved in the regulation of ECM turnover.
• 产品名称Anti-MMP14 antibody [11A-1D]
• 分子量66 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane. Melanosome
• 其它名称
  • Matrix metallopeptidase 14 (membrane inserted) antibody
  • Matrix metalloproteinase 14 antibody
  • Matrix metalloproteinase-14 antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:200-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MMP14 on human MMP14 recombinant protein using anti-MMP14 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MMP14 on HEK293 (1) and MMP14-hIgGFc transfected HEK293 (2) cell lysate using anti-MMP14 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using anti-MMP14 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-MMP14 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of HT1080 cells with MMP14 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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